储存
• 500 mL Cardiomyocyte Maintenance Medium • 100 mL Cardiomyocyte Differentiation Medium A • 100 mL Cardiomyocyte Differentiation Medium B Store at 2–8° and protect from light.

描述

The Gibco® PSC Cardiomyocyte Differentiation Kit consists of a set of serum-free and xeno-free media that enable efficient differentiation of human pluripotent stem cells (PSCs) to contracting cardiomyocytes in as few as 8 days. Unlike other methods that require multiple components and longer assay duration, the PSC Cardiomyocyte Differentiation Kit can generate cardiomyocytes from pluripotent stem cells in a ready-to-use media format and in less time.

Comprised of three 1X media that require no thawing or mixing, each medium is used consecutively over a total of 14 days, resulting in functional cardiomyocytes that express relevant physiological markers, contract in culture, and can be subsequently maintained in culture for >15 days (maintenance medium also sold separately (Cat. No. A2920801)).

No thawing or mixing required
The PSC Cardiomyocyte Differentiation Kit is comprised of 1X media that do not require frozen storage, so no thawing or mixing of reagents. Simply warm the media and add to PSC culture.

Rapid production of cardiomyocytes
The PSC Cardiomyocyte Differentiation Kit is capable of producing contracting cardiomyocytes in as few as 8 days. Differentiated cardiomyocytes can be subsequently maintained in culture for >15 days.

Generation of high quality cardiomyocytes
Cardiomyocytes generated using the PSC Cardiomyocyte Differentiation Kit are functionally relevant, expressing key markers such as TNNT2, Nkx2.5, MYH6, and α-actinin; and contract in culture.

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