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ADRA1A CHO-K1 DA ASSAY KIT 1 PLATE，K1577，Invitrogen

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订货号： 6AB2415

品牌型号： Invitrogen K1577

货期：询货期

最小订货量： 1套

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商品介绍
规格参数
包装参数

产品介绍 Product Description

规格
Accession Number:	NM_000680
Assay Entry:	Cell-based beta lactamase reporter gene
Cell Line:	CHO-K1
Cell State:	Division-Arrested Cells
Druggable Target:	GPCRs
Gene Symbol:	ADRA1A
Population Status:	Pool of Stable Cells
Product Line:	GeneBLAzer®
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	GeneBLAzer™
Target Entry:	ADRA1A
Detection Method:	Fluorescent

储存

Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
o GeneBLAzer® ADRA1A CHO-K1DA cells
o LiveBLAzer™-FRET B/G Loading Kit, 70µg
o Solution D
o GeneBLAzer® ADRA1A CHO-K1Assay Protocol
o LiveBLAzer™-FRET B/G Loading Protocol
o Certificate of Analysis
The GeneBLAzer® ADRA1A CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

描述

The GeneBLAzer® ADRA1A CHO-K1 DA (Division Arrested) cells contain the human Adrenergic Alpha-1A Receptor (ADRA1A), (Accession # NM_000680) stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line. CellSensor™ NFAT-bla CHO-K1 cells (Cat # K1078) contain a beta-lactamase (bla) reporter gene under control of the NFAT response element.

Both ADRA1A CHO-K1 DA cells are functionally validated for Z'-factor and EC50 concentrations of Phenylephrine. In addition, ADRA1A-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary in the Manuals and Brochures section. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

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