规格
Assay Entry:	Cell-based beta lactamase reporter gene
Cell State:	Dividing Cells
Druggable Target:	Cytokines, Signaling Pathway
Product Line:	CellSensor™
System Type:	CellSensor™
Target Entry:	TNF alpha, NIK, TLR, I kappa B, IRAK, IKK, NF-kappa B pathway

储存
CellSensor® Cell Lines are shipped on dry ice. Store in liquid nitrogen immediately upon receipt, or thaw for immediate use.

描述

The CellSensor® NFκB-bla RAW264.7 mouse macrophage cell line contains a beta-lactamase reporter gene under control of a stably integrated NFκB response element. This cell line is a clonal population isolated in response to lipopolysaccharide by flow cytometry. This cell line has been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time, and has been validated for Zfi-factor and EC50 concentrations of the TLR7 ligand imiquimod. Additional data using alternate stimuli are shown. The molecules responsible for coordinating the immune systems recognition of pathogens are Toll-like receptors (TLRs), of which there are currently 10 identified members in humans. TLRs are pattern recognition receptors (PRRs), which bind to pathogen-associated molecular patterns (PAMPs), small molecular sequences consistently found on pathogens. This binding activates the transcription of immune system genes and regulators such as cytokines, chemokines, and costimulatory molecules, thereby promoting the initial immune response of macrophages and neutrophils. Academic and non-profit customers, please inquire for special pricing.

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