规格
|
Assay:
|
Kinase Binding Assay |
Assay Entry:
|
Biochemical binding/kinetic |
Excitation/Emission:
|
340⁄615, 665 |
For Use With (Equipment):
|
Microplate Reader |
Format:
|
384-well plate |
Label or Dye:
|
Alexa Fluor™ 647 |
Readout:
|
Kinetic |
Shipping Condition:
|
Dry Ice |
Target Entry:
|
ABL1 G250E, ABL1 E255K, ABL1 Y253F, ABL1, PIK3CA/PIK3R1, WNK3 |
Target Enzyme:
|
Kinases (Multiple) |
Technique:
|
TR-FRET |
Validated Kinases:
|
PIK3CA, ABL1, ABL1 E255K, ABL1 G250E, ABL1 Y253F, WNK3 |
In the LanthaScreen® Eu Kinase Binding Assay, binding of an Alexa Fluor® conjugate or “tracer" such as Kinase Tracer 1710 to a kinase is detected by addition of a Eu-labeled anti-tag antibody. Binding of the tracer & antibody to a kinase results in a high degree of FRET, whereas displacement of the tracer with a kinase inhibitor results in a loss of FRET. Unlike many kinase activity assays, this assay is a simple mix and read assay, with no development steps. Learn More and See Which Kinases Have Been Validated Using This Assay