TANGO GPR119 BLA U2OS DA ASSAY 1 PLATE，K1770，Invitrogen

市场价：询价 / 套

在线询价

优惠：请询价

订货号： 5XJ8700

品牌型号： Invitrogen K1770

货期：询货期

最小订货量： 1套

+ -

加入购物车

联系我们

400-920-3909

精选商品

胰蛋白胨，500g，LP0042B，Oxoid

销售价：询价

加入购物车

商品介绍
规格参数
包装参数

产品介绍 Product Description

规格
Accession Number:	NM_178471.1
Assay Entry:	Cell-based beta-arrestin recruitment
Cell Line:	U2OS
Cell State:	Division-Arrested Cells
Cytoplasmic Tail:	Native Tail
Druggable Target:	GPCRs
Gene Symbol:	GPR119
Population Status:	Single Cell Clone Origin
Product Line:	GeneBLAzer®
Protein Family:	GPCR (G-Protein Coupled Receptors)
Readout:	End Point
Reporter Gene:	BLA (Beta-Lactamase)
System Type:	Tango™
Target Entry:	GPR119
Detection Method:	Fluorescent

储存

K1770 Tango™ GPR119-bla U2OS DA Assay Kit
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
Includes:
• Tango™ GPR119-bla U2OS DA cells
• LiveBLAzer™-FRET B⁄G Loading Kit, 70µg
• Solution D
• Tango™ GPR119-bla U2OS DA Assay Protocol
• LiveBLAzer™-FRET B⁄G Loading Protocol
• Certificate of Analysis
The Tango™ GPR119-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.

描述

The Tango™ GPR119-bla U2OS DA cells contain the human G-protein Coupled Receptor 119 (GPR119) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ GPR119-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ GPR119-bla U2OS DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

长度(mm)
宽度(mm)
高度(mm)
重量(kg)