规格
|
Assay:
|
Serine⁄Threonine Kinase Assay |
Assay Entry:
|
Biochemical phospho-specific activity (no antibody) |
Excitation/Emission:
|
405⁄465, 535 |
Label or Dye:
|
Methylcoumarin |
No. of Reactions:
|
800 Reactions |
Product Line:
|
Z´-LYTE® |
Readout:
|
End Point |
References:
|
Mol. Cell. Biol. Vol 21. pp. 88-99 (2001) |
Shipping Condition:
|
Dry Ice |
Substrate Properties:
|
Peptide-Based Substrate |
Target Entry:
|
CDK1, CDK2, CDK5 |
Target Enzyme:
|
Serine⁄Threonine Kinases |
Technique:
|
FRET |
Validated Kinases:
|
CDK1, CDK2, CDK5 |
Source:
|
CDK7 (GLAKSFGSPNRAY(pT)H) |
Format:
|
384-well plate |
储存
|
Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions. |
A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon CDK7, containing Ser-164 and a (S⁄T)-P-X-(K⁄R) consensus sequence, where X is any residue.