规格
|
Final Product Type:
|
In Vitro Transcribed RNA (Purified) |
For Use With (Application):
|
Nuclease Protection Assays, Northern Blotting |
For Use With (Equipment):
|
Vacuum Manifold, Centrifuge |
Format:
|
Tube(s) |
Isolation Technology:
|
Silica Spin Column |
No. of Reactions:
|
20 Preps |
Sample Type:
|
In Vitro Transcribed RNA, Total RNA |
Yield:
|
500 µg |
High-throughput Compatibility:
|
Not High-throughput Compatible (Manual) |
Purification Target:
|
In Vitro Transcribed RNA |
Purification Time:
|
30 min |
Shipping Condition:
|
Room Temperature |
Starting Material Amount:
|
1 ng to 500 µg of dsRNA>200b or ssRA>100bp |
Product Line:
|
Ambion™, MEGAclear™ |
储存
|
Binding Solution, Wash Solution Concentrate, and 5 M Ammonium Acetate are stored at 4°C. Elution Solution can be stored at any temperature, and Filter Cartridges and Collection and Elution Tubes are stored at room temperature. |
The MEGAclear™ Kit is designed for the quick cleanup of any large-scale transcription reaction. The MEGAclear™ Kit efficiently separates RNA from unincorporated NTPs, enzymes, and buffer components. Advantages of the MEGAclear™ Kit:
• Purifies transcription reactions to produce high-quality RNA, free of unincorporated NTPs, enzymes, and buffer components
• High recovery from 1 ng to 500 µg of input
• Ideal for purification of aRNA amplification reactions, nonisotopically labeled RNA probes, and capped RNA transcripts for oocyte injections
• Quick protocol—purify RNA in less than 30 minutes
Using the MEGAclear™ Kit
The protocol is quick and easy. The transcription reaction is mixed with Binding Solution, passed over an RNA-binding filter, and washed; an elution step releases the purified RNA, which is now ready for any application requiring highly pure synthetic or in vitrotranscribed RNA. For RNA recovery of 70% or greater, single-stranded RNA should be >100 nt and double-stranded RNA should be >200 bp.