规格
Assay Category:	Biochemical Assay
Druggable Target:	Nuclear Receptors
Label or Dye:	Tb (Terbium)
Product Line:	LanthaScreen®
Readout:	TR-FRET (Activity)
Technique:	TR-FRET
Validated Application:	Nuclear Receptor Assay
Color:	Green
Detection Method:	Fluorescent
For Use With (Equipment):	Microplate Reader
Format:	384-well plate

储存
Tb-anti-GST Antibody (Rabbit): Store at -20 °C CAR-LBD, GST: Store at -80 °C Fluoroscein Coactivator Peptide: Store at -20 °C TR-FRET Coregulatore Buffer G: Store at 4 °C 1M DTT: Store at -20 °C or -80 °C

描述

This kit contains Rabbit Tb-Anti-GST antibody; other kit components are the same as kit PV4836:

The LanthaScreen® TR-FRET Constitutive Androstane Receptor (CAR) Coactivator Assay provides a sensitive and robust method for highthroughput screening (HTS) of potential CAR ligands as agonists of ligand-dependent coactivator recruitment or inverse agonists of liganddependent coactivator displacement. The kit uses a terbium-labeled anti-GST antibody, a fluorescein-labeled coactivator peptide, and a CAR ligand-binding domain (CAR-LBD) that is tagged with glutathione-S-transferase (GST) in a homogeneous mix-and-read assay format.

To assay:
When running the LanthaScreen® TR-FRET CAR Coactivator Assay, CAR-LBD is added to ligand test compounds followed by addition of a mixture of the fluorescein-coactivator peptide and terbium anti-GST antibody. After an incubation period at room temperature, the 520 nm/ 495 nm TR-FRET ratio is calculated and can be used to determine the EC50 from a dose response curve of the compound. Based on the biology of the CAR-coactivator peptide interaction, this ligand’s EC50 is a composite value representing the amount of ligand required to bind to receptor, effect a conformational change, and either recruit or displace coactivator peptide (Figures 1 and 2).

长度(mm)
宽度(mm)
高度(mm)
重量(kg)