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The Naive CD8+ T Cell Isolation Kit was developed for the isolation of naive CD8+ T cells from human PBMCs in a two-step procedure.
The isolation of naive CD8+ T cells is performed in a two-step procedure. First, naive T cells are enriched by depletion of non-naive T cells and NK cells, which are indirectly magnetically labeled with a cocktail of biotin-conjugated antibodies and Anti-Biotin MicroBeads. In a second step, the enriched naive T cells are labeled with CD8 MicroBeads for subsequent positive selection of the CD8+ naive T cells.
Naive CD8+ T cells isolated with the Naive CD8+ T Cell Isolation Kit can be used for functional T cell studies, e.g., studies on signal transduction or cytokine expression after activation of the naive CD8+ T cells.
For the first magnetic separation (depletion): LD or autoMACS® Columns. For the second magnetic separation (positive selection): MS or autoMACS Columns.
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