规格
|
Assay:
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β-Gal (lacZ) Assay |
Detection Method:
|
Colorimetric |
Substrate:
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X-Gal |
Substrate Properties:
|
Chemical Substrate |
Substrate Type:
|
Beta-Gal Substrate |
Target Enzyme:
|
Beta-Galactosidase |
储存
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The β-Gal Staining Kit contains sufficient reagents to stain fifty 60 mm plates. The kit includes 10X PBS (phosphate buffered saline), X-gal, staining solutions A, B, and C, 10X fixative solution, and 10 µg of pcDNA™ 3.1/His/lacZ control vector. Store the 10X PBS at room temperature. All other components should be stored at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
The β-Gal Staining Kit allows you to determine the percentage of transfected cells expressing lacZ. lacZ is a bacterial gene often used as a reporter construct in eukaryotic transfection experiments. The gene product of lacZ, β-galactosidase, is resistant to proteolysis in cellular lysates and its activity is easily assayed. β-galactosidase catalyzes the hydrolysis of X-gal, producing a blue color that can be easily visualized under a microscope.