• Resuspension buffer, store at 4°C upon reciept
• Lysis buffer, store at room temperature
• Precipitation buffer, store at room temperature
• Binding buffer, store at room temperature
• Wash buffer 1, store at room temperature
• Wash buffer 2 (concentrate), store at room temperature
• Elution buffer, store at room temperature
• Column assemblies, store at room temperature
• Syringe filters, store at room temperature
• Syringe plungers, store at room temperature
• Collection tubes, store at room temperature

描述

The PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/µg) plasmid DNA in about 30 minutes. The simple precipitation-free protocol yields up to 1.5 mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.

NOTE: The use of a vacuum manifold is required to perform this procedure. We recommend our FastVac Vacuum Manifold.

Advantages of the PureLink Fast Low-Endotoxin Maxi Plasmid Purification Kit include:
• Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
• Low endotoxin plasmid—the resulting plasmid contains <1 EU/µg endotoxins
• High yields—isolate up to 1.5 mg of high-quality plasmid DNA
• Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization

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