规格
Accession Number:	NP_002871, Y340D, Y341D
Assay Entry:	Biochemical terbium phospho-specific activity, Biochemical binding/kinetic
Kinase Group:	Ser⁄Thr Kinases: TKL Group
Shipping Condition:	Dry Ice
System Compatibility:	LanthaScreen® Antibody, LanthaScreen® Substrate, Z'-LYTE™, Omnia® Bulk Substrate
Target Entry:	RAF1 (cRAF) Y340D Y341D
Target Gene:	v-raf-1 murine leukemia viral oncogene homolog 1
Expression System:	Baculovirus
Protein Family:	Kinases & Inhibitors
Protein Form:	Recombinant, Catalytic
Protein Subtype:	Serine⁄Threonine Kinases
Research Category:	Signal Transduction
Species:	Human
Validated Application:	Kinase Assay
Gene ID (Entrez):	4773, 5894
Molecular Weight:	66.4 kDa
Quantity:	10μg
Gene Alias:	RAF1 (cRAF) Y340D Y341D
KinaseFamily:	RAF Kinase Family
Protein Length:	aa 306-648; Y340D, Y341D
Protein Tag:	GST Tag

储存
Storage Buffer: 50mM Tris, pH 7.5, 150mM NaCl, 0.5mM EDTA, 0.02% Triton X-100, 2mM DTT, 50% glycerol Store at -80°C. At first use, aliquot and store at -80°C to avoid multiple freeze-thaws.

描述

RAF1 (cRAF) is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylates and activates the serine⁄threonine specific protein kinases ERK1 and ERK2. The RAF1 Y340D Y341D mutant is constitutively active.

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