规格
Biological Activity:	ED₅₀ range = 0.1-1.0 ng⁄mL
Endotoxin Level:	< 0.1 ng/µg
Form:	Liquid
Indicator Cell:	Balb/3T3 cells
Mass:	100 µg
Product Line:	Gibco®
Protein Form:	Recombinant, Ligand, Truncated
Protein Length:	aa 10-155
Protein Tag:	Untagged
Purity or Quality Grade:	Carrier-Free
Reactivity:	Human
Research Category:	Neurobiology, Oncology, Stem Cell Research, Microbiology, Angiogenesis
Shipping Condition:	Dry Ice
Specific Activity:	1.0 x 10⁷ - 1.0 x 10⁶ units⁄mg
Molecular Weight:	17 kDa
Purity:	95 %
Gene ID (Entrez):	FGF2
Purification Method:	Sequential Chromatography
Species:	Human
Protein Family:	Growth Factors & Receptors
Protein Subtype:	FGFs (Fibroblast Growth Factors)
Gene Alias:	FGF-Basic (AA 10-155)
Accession Number:	NM_002006
Validated Application:	Cell Culture
Expression System:	E. coli

储存
Store at ≤ 20°C. Upon initial thawing, we recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. The contents should then be apportioned into working aliquots and stored at ≤ 20°C. Avoid repeated freeze⁄thaw cycles. Further dilutions should be made in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.

描述

FGF-Basic (AA 10-155) is a bioactive protein intended for use in cell culture applications. Fibroblast growth factor-basic is known as FGF-basic, bFGF, FGF 2, FGF-β, and heparin binding growth factor. FGF-basic is involved in a number of biological processes, including embryonic development and differentiation, neuron differentiation, survival, regeneration, and the proliferation of cells of mesodermal origin and many cells of neuroectodermal, ectodermal, and endodermal origin.

Features of recombinant human FGF-basic:

• High purity—no interference from other proteins or contaminants
• High biological activity—more results with less protein
• Proven compatibility—proteins bioassayed with Gibco® media

High-purity, recombinant, bioactive protein
Human FGF-basic (AA 10-155) is one of our more than 250 available recombinant proteins. To help ensure our growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High biological activity
In-house activity testing of recombinant proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant human FGF-basic specific activity is determined by the dose dependent proliferation of BALB/3T3 cells.

Proven compatibility
Our recombinant proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

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