CST NOSPIN PLASMID MICRO KIT 96，CS10201，Invitrogen 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

规格
Final Product Type:	Plasmid DNA
For Use With (Application):	Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Purification Target:	Plasmid DNA
Sample Type:	Bacterial Culture
Isolation Technology:	ChargeSwitch® Chemistry
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Prep Scale:	< 100 µg (Small-Scale) Plasmid DNA
Shipping Condition:	Room Temperature
Product Line:	ChargeSwitch®

储存

All components of the ChargeSwitch® NoSpin Plasmid Kits are shipped at room temperature. Upon receipt, store as follows: Store ChargeSwitch® Precipitation Buffer (N5) at 4“C. Mix the RNase I in ChargeSwitch® Resuspension Buffer (R4) and store the resulting solution at 4“C. Store all other components at room temperature. All components are guaranteed stable for 6 months if stored properly.

Components:

ChargeSwitch® MagnaClear™ Beads
ChargeSwitch® Magnetic Beads
ChargeSwitch® Resuspension Buffer (R4; 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)
ChargeSwitch® Lysis Buffer (L9)
ChargeSwitch® Precipitation Buffer (N5)
ChargeSwitch® Wash Buffer (W11)
ChargeSwitch® Wash Buffer (W12)
ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
RNase A (5 mg/ml in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)

描述

Sample size 0.5-1 ml overnight culture; Typical yield up to 5 µg

• Provides centrifuge-free purification, ideal for automation
• Avoids introduction of enzymatic contaminants for better results
• Optimized for automation

The ChargeSwitch® NoSpin Kits provide centrifuge-free microprep plasmid purification, ideal for automation. The novel NoSpin reagent causes bacterial cell flocculation and allows cells to be pelleted without the need for automation bottlenecks such as centrifugation. This kit includes all the necessary reagents for the number of preps listed and requires the use of a magnetic accessory. Please see the Accessories Chapter (Chapter 6) to select the best magnetic accessory for your needs.

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