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MAX EFF DH10B COMP CELLS 1ML #18297,18297010,Invitrogen

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订货号 5RB7914
品牌型号 Invitrogen 18297010
货期 询货期
最小订货量 1套
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产品介绍 Product Description
规格
Bacterial or Yeast Strain: DH10B
Blue/White Screening: Yes
Cloning Methylated DNA: Yes
Cloning Unstable DNA: Not Suitable for cloning unstable DNA
Competent Cell Type: Chemically Competent
F'episome (to make ssDNA): Lacks F' Episome
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Preparing Unmethylated DNA: Not Suitable for preparing unmethylated DNA
Product Line: DH10B, MAX Efficiency™
Propagating ccdB Vectors: Not for ccdB vector propagation
Reduces Recombination: Yes
Shipping Condition: Dry Ice
T1 Phage - Resistant (tonA): No
Transformation Efficiency: > 1x10^9
Transformation Efficiency Level: High Efficiency (> 10^9 cfu⁄µg)
Format: Tube(s)
Improves Plasmid Quality: Yes
Species: E. coli
储存
Contains:
• MAX Efficiency DH10B Competent Cells: 5 vials, 200 µl each (total of 1 ml)
• pUC19 DNA (0.01 µg/ml): 1 vial, 100 µl
• S.O.C. medium: 2 bottles, 6 ml each

Store Competent Cells at -80°C. Store pUC19 DNA at -20°C. Store SOC Medium at 4°C or at room temperature.
描述

MAX Efficiency DH10B Competent Cells are highly efficient E. coli, perfect for most applications. DH10B E. coli are available in both electrocompetent and chemically competent formats. The high transformation efficiency makes them ideal for generating cDNA or genomic libraries. High-efficiency transformation is seen with plasmids 150 kb in size. The versatile DH10B genotype has the following features:

lacZ for blue/white screening of recombinant clones
• Elimination of mcrA, mcrBC, mrr, and hsdRMS restriction systems to allow construction of more representative genomic libraries
• A1 mutation to increase plasmid yield and quantity

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