PCDNA6.2/C-EMGFP-GW/TOPO 20 RXNS，K35920，Invitrogen 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

规格
Cloning Method:	TOPO® with Gateway®
Key Functions:	Reporter Assays, Subcellular Localization
Product Line:	Gateway®, TOPO®, Vivid Colors™, pcDNA™
Constitutive or Inducible System:	Constitutive
Delivery Type:	Transfection
Promoter:	CMV
Reporter Gene:	GFP (EmGFP)
Selection Agent (Eukaryotic):	Blasticidin
Vector:	pcDNA
Protein Tag:	GFP (EmGFP)

储存

Each pcDNA™6.2-TOPO® Expression Vector Kit contains two boxes. The TOPO® box contains linearized and topoisomerase I-activated pcDNA™6.2-TOPO® vector, control template, primers for sequencing, PCR reaction components, and an expression control plasmid. The TOPO® box should be stored at -20°C. The One Shot® box contains transformation reagents including single-use 50 µl aliquots of One Shot® TOP10 chemically competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control. Store competent E. coli at -80°C. All reagents are guaranteed stable for 6 months when properly stored.

描述

The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO® Expression Vectors (Figure 1) allow you to rapidly clone your gene and fuse it to the widely used and well characterized Fluorescent Proteins (FPs) from the jellyfish Aequorea victoria (1, 2). These powerful TOPO® cloning vectors contain the Emerald Green Fluorescent Protein (EmGFP) or the Yellow Fluorescent Protein (YFP) for simple, non-invasive detection of recombinant protein (Figure 2). Both FPs have been humanized for optimal mammalian expression (3). The Vivid Colors™ pcDNA™6.2 Fluorescent Protein TOPO® Expression Vectors offer:
• Topoisomerase I for one-step, 5-minute TOPO® cloning of your PCR-amplified gene of interest
• CMV promoter for high-level expression of the recombinant fluorescent fusion protein
• Ability to fuse EmGFP or YFP to the N- or C-terminus of your protein
• Bsd resistance marker for rapid selection of stable cell lines

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