- 商品介绍
- 规格参数
- 包装参数
|
规格 |
|
| Enzyme: | CIP |
| Shipping Condition: | Approved for shipment on Wet or Dry Ice |
| Concentration: | 20U/μL |
|
储存 |
|
| Calf Intestinal Alkaline Phosphatase (20 units/µL) is supplied with a vial of 10X dephosphorylation buffer [500 mM Tris-HCl (pH 8.5), 1 mM EDTA], vial of dilution buffer [50% glycerol, 25 mM Tris-HCl (pH 7.6), 1 mM MgCl2 , and 0.1 mM ZnCl2 ]. Store Calf Intestinal Alkaline Phosphatase at -20°C. |
|
描述 |
Calf Intestinal Alkaline Phosphatase (CIAP) is a phosphomonoesterase that removes 3´ and 5´ phosphates from DNA and RNA.
Concentration: 20 units/µL
Applications: Dephosphorylation of 5´-phosphorylated termini of vector DNA to prevent self-ligation (1). Dephosphorylation of 5´ termini of nucleic acids prior to forward reaction with kinase.
Source: Purified from calf intestinal mucosa.
Performance and Quality Testing: Endodeoxyribonuclease, 3´ exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay.
Unit Definition: One unit hydrolyzes 1 µmol of 4-nitrophenyl phosphate in 1 min. at 37°C.
Unit Reaction Conditions: 1 M diethanolamine buffer, 10 mM 4-nitrophenyl phosphate, 0.25 mM MgCl2 (pH 9.8) in 900 µl for 10 min. at 37°C.
| 长度(mm) | |
| 宽度(mm) | |
| 高度(mm) | |
| 重量(kg) |
-
询价
-
询价
-
询价
-
¥ 749.00
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
询价
-
¥ 58184.19
-
¥ 21144.84
-
¥ 108621.02
-
询价
-
询价
-
询价
-
询价
微信小程序
7X24小时在线咨询