规格
|
Cell Permeability:
|
Cell-Permeant |
Detection Method:
|
Fluorescent |
For Use With (Equipment):
|
Fluorescence Microscope, Flow Cytometer, Fluorometer, Fluorescent Imager |
Indicator:
|
Calcium Indicator |
Indicator Properties:
|
Fluorescent Dye-Based |
Shipping Condition:
|
Room Temperature |
储存
|
Store in freezer (-5 to -30°C) and protect from light. |
The Ca2+ ionophore A-23187 is commonly used for in situ calibrations of fluorescent Ca2+ indicators, to equilibrate intracellular and extracellular Ca2+ concentrations and to permit Mn2+ to enter the cell to quench intracellular dye fluorescence. Although the intrinsic fluorescence of A-23197 is too high for use with UV-excitable calcium indicators like fura-2, indo-1 and quin-2 it is suitable for use with the visible light-excitable indicators, including fluo-4, fluo-3, Calcium Green and rhod-2. A-23187 can also be used to equilibrate extracelular and intrcellular Mg2+ concentrations, making it useful for calibrating Mg2+ indicators.