规格
Cloning Method:	Gateway®
Product Line:	Gateway®
Promoter:	Polyhedrin
Vector:	pDEST, Gateway Baculovirus Vectors
Protein Tag:	GST Tag, His Tag (6x), Untagged

储存

The Baculovirus Expression System with Gateway® Technology includes LR Clonase® enzyme mix and reaction buffer, destination vectors pDEST™8, pDEST™10, and pDEST™20, proteinase K solution (2 µg/µl), pENTR™-gus positive control, and Library Efficiency® DH5Competent Cells. The kit does not include MAX Efficiency® DH10Bac™ Competent Cells. Store LR Clonase® enzyme mix and Competent Cells at -80°C. Store all other components at -20°C. Components are guaranteed stable for 6 months when properly stored.

描述

The Baculovirus Expression System with Gateway® Technology is designed to create recombinant pFastBac™ plasmids containing the polyhedrin promoter. The Baculovirus Expression System with Gateway® Technology offers:

• Expression using the Bac-to-Bac® technology (1)
• Destination vectors carrying the polyhedrin promoter for production of native (pDEST™8), N-terminal histidine fusion (pDEST™10), and N-terminal GST fusion (pDEST™20) proteins
• pDEST™10 vector containing a TEV protease cleavage site for removal of the fusion tag after protein purification (2)

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