规格
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Column Type:
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Affinity Column |
Shipping Condition:
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Wet Ice |
Stationary Phase:
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Protein A |
Format:
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Suspension |
储存
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Protein A Agarose is supplied as a 45% to 55% suspension in 20 mM sodium phosphate (pH 7.2), 0.15 M NaCl, 0.02% (w/v) merthiolate. Store at +4°C. |
Protein A Agarose is suitable for purifying IgG molecules from a variety of species, fractionation of IgG fragments, fractionation of cells tagged with IgG, and isolation of antigen-antibody complexes. In addition, Protein A interacts with some classes of IgM and IgA. The matrix consists of purified Protein A covalently attached to cross-linked 4% agarose beads using the cyanogen bromide method. Protein A Agarose can bind >18 mg of human IgG/mL of gel.
Performance and quality testing
Binding capacity for human IgG is determined by affinity chromatography.