StraightFrom Buffy Coat REAlease CD4 MicroBead Kit, human，130-127-149，美天旎

The StraightFrom^® Buffy Coat CD4 REAlease^® MicroBead Kit has been developed for the rapid positive selection of bead-free or label-free CD4⁺ cells, starting directly from one buffy coat using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS^® Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. Whole Blood Columns are included in the kit to allow direct processing of an entire buffy coat with a simple and quick protocol.

CD4 is an accessory molecule in the recognition of MHC class II/peptide complexes by the TCR heterodimers on CD4⁺ T helper cells. CD4 is expressed on T helper cells and at a lower level on monocytes and dendritic cells. The CD4 molecule is the receptor for the human immunodeficiency virus. The CD4 antibody recognizes most thymocytes and about 65% of all peripheral blood T cells.

One entire buffy coat is labeled with StraightFrom^® Buffy Coat REAlease^® CD4-Biotin Complex followed by Anti-Biotin MicroBeads and loaded onto Whole Blood Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus. After removing the column from the magnetic field, the magnetically retained CD4⁺ cells are eluted as the positively selected cell fraction bead-free, or free of MicroBeads. Optionally, the REAlease Biotin Complex can be removed for label-free target cells.

The purified CD4⁺ cells are bead-free, practical for consecutive magnetic isolation and optionally label-free with epitopes available for any downstream application. The isolated cells are suited for further flow cytometric analyses, molecular biology applications, and functional studies.