PepTivator® Uveal Melanoma GNAQ_Q209P, premium grade，130-135-447，美天旎

PepTivator Uveal Melanoma GNAQ_Q209P is a pool of lyophilized peptides, consisting of 15-mer, 10-mer, and 9-mer sequences containing the Q209P mutation of human Guanine nucleotide-binding protein subunit alpha-q (UniProt ID: P50148). Q209P is an important driver mutation that occurs with high frequency in Uveal melanoma.

PepTivator Uveal Melanoma GNAQ_Q209P covers the mutated protein domain [DLQSVIFRMVDVGGPRSERRKWIHCFENV] and contains every 15-mer, 10-mer, and 9-mer peptide bearing the mutated amino acid. This diversity allows loading of HLA without prior knowledge of alleles.

PepTivator Uveal Melanoma GNAQ_Q209P was developed for the efficient in vitro stimulation of Q209P–specific CD4⁺ and CD8⁺ T cells. Peptides of 15-, 10-, and 9 amino acids in length, represent an optimal solution for stimulating both CD4⁺ and CD8⁺ T cells in various applications, as well as providing the best coverage of potential HLA alleles.

Quantitative, phenotypical, or functional analysis of Q209P–specific T cell immunity can provide important information on the natural course of immune responses.

Uveal melanoma is a critical type of eye cancer that can lead to high rate of metastasis, especially in the liver. There are various strategies developed to treat the metastatic disease, like a T cell Receptor (TCR)-based immunotherapy. There are significant discoveries in the signalling pathways that are altered in uveal melanoma, like there are around 90% of patients with mutually exclusive activating mutations in the GNAQ and GNA11 genes. Results suggest a high immunogenicity potential of a GNAQ and GNA11 variants that could allow the generation of novel treatments to cure Uveal Melanoma like neoantigen vaccinations.

The PepTivator Peptide Pools have been specially developed for efficient in vitro stimulation of antigen-specific CD4⁺ and CD8⁺ T cells, as peptides of 15 amino acid length with 11 amino acid overlap represent the optimized solution for stimulating both CD4⁺ and CD8⁺ T cells in various applications. Stimulation of T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and upregulation of activation markers, which then allows the detection or isolation of antigen-specific T cells. Quantitative, phenotypical, or functional analysis of antigen-specific T cell immunity can provide important information on the natural course of the natural course of immune responses.