- 商品介绍
- 规格参数
- 包装参数
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规格 |
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| Cloning Method: | Restriction Enzyme ⁄ MCS |
| Product Line: | DES® |
| Protein Tag Position (to your gene): | C-terminal |
| Promoter: | MT |
| Vector: | pMT |
| Protein Tag: | His Tag (6x), V5 Epitope Tag |
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储存 |
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• 20 µg each of pMT/V5-His A, B, and C (0.5 µg/µl in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 in a volume of 40 µl) • 20 µg pMT/V5-His/lacZ (0.5 µg/µl in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 in a volume of 40 µl) Store at -30°C to -10°C. |
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描述 |
The DES®-Inducible Kit provides the expression vector pMT/V5-His for expression of recombinant proteins. This vector uses the Drosophila metallothionein gene promoter that is induced in S2 cells upon addition of copper sulfate or cadmium chloride to the culture medium.
The pMT/V5-His vector offers the following features:
• Small size (3.5 kb) to improve DNA yields and increase subcloning efficiency
• C-terminal V5 epitope tag for rapid detection with Invitrogen's Anti-V5 Antibody
• C-terminal 6xHis tag for simple purification of recombinant fusion proteins using nickel-chelating resin
To facilitate cloning, a set of three vectors-A, B, and C-is provided. Each vector has the multiple cloning site in a different reading frame relative to the coding sequence of the C-terminal tag.
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¥ 91259.08
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¥ 66363.43
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¥ 29754.56
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