规格
Exonuclease Activity:	5' - 3'
Fidelity (vs. Taq):	1 X
GC-Rich PCR Performance:	Low
Hot Start:	Built-In Hot Start
Includes:	5 boxes of AmpliTaq™ Gold DNA polymerase, for a total of 25,000 units (25 x 1000 units). Each box contains 5 tubes x 200μL each of AmpliTaq Gold DNA Polymerase (at 5U/μL), 1000 units total per tube. Each box also contains 5 tubes x 1.5mL of Buffer I (100mM Tris-HCl, pH 8.3, 500mM KCl, 15mM magnesium chloride, 0.01% (w/v) gelatin).
Overhang:	3'-A
Polymerase:	AmpliTaq Gold DNA Polymerase
Product Line:	AmpliTaq Gold™
Quantity:	25 x 1000 units
Reaction Format:	Separate Components
Reaction Speed:	Standard
Size (Final Product):	5 kb or less

储存
• 25 x 1000 U AmpliTaq Gold DNA Polymerase • 100 x 1.5 mL 10X PCR Buffer I containing MgCl₂ Store at –15 to –30°C.

描述

AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase requiring thermal activation. Features of this enzyme:

• Automated chemical hot-start enzyme for increased specificity, sensitivity, and yield
• Time-released thermal activation improves sensitivity in low copy number amplifications
• Successful multiplex PCR saves time and reagents
• Includes GeneAmp® 10X PCR Buffer I containing 15 mM MgCl₂

Hot-start activation
The modified enzyme is provided in an inactive state. Upon thermal activation, the modifier is permanently released, regenerating active enzyme. The resulting hot-start PCR amplification provides increased sensitivity, specificity, and yield over conventional PCR techniques.

Higher specificity, higher yield
AmpliTaq Gold® DNA Polymerase can be activated partially or completely in a pre-PCR heat step, or can be allowed to activate slowly in a time-released manner during the denaturation steps of thermal cycling. With or without a limited up-front heat activation step, active enzyme is released slowly during thermal cycling to match template concentration and increase specificity. The yield of specific product increases because reactants are not wasted in the formation of unintended products. Because AmpliTaq Gold® DNA Polymerase is a chemical hot-start enzyme, there is no worry of biological contamination.

AmpliTaq Gold® 360 DNA Polymerase for superior performance
Also available is AmpliTaq Gold® 360 DNA Polymerase for even better PCR performance. Compared to AmpliTaq Gold® DNA Polymerase, AmpliTaq Gold® 360 DNA Polymerase is purified by an additional proprietary separation process to eliminate contaminating bacterial DNA sequences from the enzyme preparation. When used with the enhanced 360 Gold Buffer, this ultra-pure enzyme, in addition to its hot-start capabilities, reduces false positive results, amplifies low-level target sequences, and promotes the amplification of a variety of templates. Also included with this product is a 360 GC Enhancer to work through challenging GC-rich templates.

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