规格
Cloning Method:	Gateway®
Key Functions:	Reporter Assays, Viral Expression
Product Line:	Gateway®, ViraPower™, Vivid Colors™
Selection Marker Promoter:	PGK Promoter
Constitutive or Inducible System:	Constitutive
Delivery Type:	Lentiviral
Promoter:	CMV
Reporter Gene:	GFP (EmGFP)
Selection Agent (Eukaryotic):	Blasticidin
Vector:	pLenti
Protein Tag:	Untagged

储存
pLenti6.2-GW⁄EmGFP Vector: 20 µg of control vector is supplied in solution as 40 µl of 0.5 µg⁄µl in 10 mM Tris–HCl, 1 mM EDTA, pH 8.0. Store at –20°C.

描述

The Vivid Colors™ pLenti6.2-GW⁄EmGFP Expression Control Vector is a ViraPower™ positive control lentiviral vector containing Emerald Green Fluorescent Protein (EmGFP). It is designed for use with the ViraPower™ Lentiviral Expression Systems as a positive control to enable the detection of EmGFP fluorescence following transfection in 293FT cells, as a titer control to produce an EmGFP-expressing lentivirus stock and as a transduction control following transduction in both dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of EmGFP and the PGK promoter for driving long-term, persistent expression of the Blasticidin stable selection marker. This is not a cloning vector.

Advantages
• Optimization of viral transduction efficiency
• Optimization of 293FT Transfection
• 2 day titer of functional virus using EmGFP

Key Features
• Human cytomegalovirus (CMV) immediate early promoter to control high-level expression of the EmGFP
• PKG Promoter for expression of Blasticidin selection marker

Kit Includes
pLenti6.2-GW⁄EmGFP Vector

Related SKUs
• 293FT Cell Line (R70007; R70007)
• ViraPower™ Lentiviral Support Kit (K4970-00)
• ViraPower™ Lentiviral Packaging Mix (K4975-00)
• Lipofectamine® 2000 (11668-019; 11668-027)

For research use only. Not intended for any therapeutic or diagnostic use.

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