MEDIUM 199 500ML，11043023，Invitrogen

规格
Classification:	Animal Origin
Culture Environment:	CO2
HEPES Buffer:	No HEPES
Osmolality:	270 - 310 mOsm/kg
Product Line:	Gibco®
Shipping Condition:	Room Temperature
Sodium Bicarbonate Buffer:	Sodium Bicarbonate
Sodium Pyruvate Additive:	No Sodium Pyruvate
Supplementation Required:	Serum
Volume (Metric):	500 ml
Form:	Liquid
Glutamine:	L-Glutamine
Phenol Red Indicator:	No Phenol Red
pH Range:	7.1 - 7.4
Concentration:	1 X

储存

Storage conditions: 2-8° C. Protect from light. Shipping conditions: Ambient. Shelf life: 12 months from date of manufacture.

描述

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. We offer a variety of Gibco® Medium 199 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

The complete formulation is available.

Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO₂ incubator, or with Hanks' salts for use without CO₂.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® Medium 199 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO₂ environment to maintain physiological pH.