The StraightFrom^® Leukopak^® CD3 MicroBead Kit has been developed for the rapid positive selection of CD3⁺ cells directly from a ½ Leukopak^® using the MultiMACS™ Cell24 Separator Plus for semi-automated separation or a MACS^® Separator for manual separation. No sample preparation is required, including density gradient centrifugation, erythrocyte lysis, wash steps, or cell count. Multi-24 Column Blocks and Whole Blood Column Elution Buffer are included in the kit to allow direct processing of a ½ Leukopak^®.

Background information

The CD3 antigen is associated with the T cell receptor (TCR) heterodimer. CD3 is expressed on all T cells, constituting 15–20% of human peripheral blood leukocytes.

Detailed separation procedure

A ½ Leukopak^® is labeled with StraightFrom^® Leukopak^® CD3 MicroBeads and loaded onto Whole Blood Columns, which are placed in the magnetic field of a MultiMACS™ Cell24 Separator Plus or a MACS^® Separator. After removing the column from the magnetic field, the magnetically retained CD3⁺ cells can be eluted as the positively selected cell fraction.

Downstream applications

The purified CD3⁺ cells are well suited for further flow cytometric, functional, or molecular analysis.

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