DMEM NUTRIENT MIX F12 500ML，21041025，Invitrogen

规格
Glucose:	High Glucose
HEPES Buffer:	No HEPES
Product Line:	Gibco®
Serum Supplementation:	Standard Serum Supplementation
Shipping Condition:	Room Temperature
Sodium Pyruvate Additive:	Sodium Pyruvate
Volume (Metric):	500 ml
Form:	Liquid
Glutamine:	L-Glutamine
Phenol Red Indicator:	No Phenol Red

储存

Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture

描述

DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

The complete formulation is available.

Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO₂ environment to maintain physiological pH.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture DMEM/F-12 at two separate facilities, located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP-manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.