ELECTRO,TOP10 400 UL，C66455，Invitrogen 供应商、价格、厂家、报价、规格参数

产品介绍 Product Description

规格
Bacterial or Yeast Strain:	TOP10
Blue/White Screening:	Yes
Cloning Methylated DNA:	Yes
Cloning Unstable DNA:	Not Suitable for cloning unstable DNA
Competent Cell Type:	Electrocompetent
F'episome (to make ssDNA):	Lacks F' Episome
Format:	Tube(s)
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Improves Plasmid Quality:	Yes
Includes:	Contains enough electrocompetent cells for 12 x 40μL rxns or 25 x 20μL rxns
Plasmid:	May be used for plasmids > 20 kb
Preparing Unmethylated DNA:	Not Suitable for preparing unmethylated DNA
Product Line:	Electrocomp™
Propagating ccdB Vectors:	Not for ccdB vector propagation
Quantity:	5 x 100 μL
Reduces Recombination:	Yes
Species:	E. coli
T1 Phage - Resistant (tonA):	No
Transformation Efficiency:	> 1x10^10
Transformation Efficiency Level:	High Efficiency (> 10^9 cfu⁄µg)

描述

TOP10 E. coli are provided at a transformation efficiency of 1 × 10¹⁰ cfu/µg supercoiled DNA and are ideal for high-efficiency cloning and plasmid propagation. They allow stable replication of high-copy number plasmids.

The genotype of TOP10 cells is similar to the DH10B™strain, and offers the following features:

• hsdR for efficient transformation of unmethylated DNA from PCR amplifications
• mcrA for efficient transformation of methylated DNA from genomic preparations
• lacZΔM15 for blue/white color screening of recombinant clones
• endA1 for cleaner preparations of DNA and better results in downstream applications due to the elimination of non-specific digestion by Endonuclease I
• recA1 for reduced occurrence of non-specific recombination in cloned DNA

One Shot® TOP10 Kits are available with either chemically competent or electrocompetent E. coli to fit your specific transformation needs. TOP10 E. coli are also available in the high-throughput MultiShot™ format.

Note: This kit contains enough electrocompetent cells for 12 x 40 µL rxns or 25 x 20 µL rxns. Please see the product manual for reaction scale recommendations.

Genotype:
F^- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara-leu)7697 galU galK rpsL endA1 nupG

长度(mm)
宽度(mm)
高度(mm)
重量(kg)