规格
Sample Type:	RNA
Detection Method:	Gel Electrophoresis
No. of Reactions:	100
Size (Final Product):	14 kb or less
Hot Start:	Built-In Hot Start
PCR Method:	1-Step RT-PCR
Polymerase:	Platinum™ SuperFi™ DNA Polymerase
Reverse Transcriptase:	SuperScript™ IV
Shipping Condition:	Dry Ice
Product Line:	SuperScript™

储存
• 50 µL SuperScript IV RT Mix • 2 x 1.25 mL 2X Platinum SuperFi RT-PCR Master Mix • 100 µL ezDNase Enzyme • 100 µL 10X ezDNase Buffer • 2 x 1.25 mL nuclease-free water Store at -20°C in a non-frost-free freezer.

描述

The Invitrogen SuperScript IV One-Step RT-PCR System with ezDNase combines the high processivity of SuperScript IV Reverse Transcriptase (RT) with the high-fidelity of Platinum SuperFi DNA Polymerase to provide unmatched one-step RT-PCR performance. With this convenient one-step formulation and gene-specific primers, you can perform both cDNA synthesis from total RNA or mRNA and PCR amplification in a single tube.

Features of the SuperScript IV One-Step RT-PCR System with ezDNase include:
• Superior performance with sensitivity down to 0.01 pg RNA, target length up to 13.8 kb, and the fastest one-step RT-PCR protocol
• Reliable target detection even with suboptimally pure RNA samples
• Two-phase hot-start activation mechanism for high sensitivity, specificity, and room temperature setup
• Fast and easy gDNA removal for superior accuracy and confidence in results

SuperScript IV RT is a proprietary MMLV RT mutant with increased processivity, thermostability, and inhibitor resistance, as well as reduced RNase H activity, for sensitive and efficient full-length cDNA synthesis. Platinum SuperFi DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with trusted Platinum hot-start technology for the highest success in PCR. The combination of these two engineered enzymes produces enhanced RT-PCR yields and fidelity, as well as amplification of longer templates compared to other one-step RT-PCR kits. Inclusion of ezDNase enzyme for use upstream of the RT step further accelerates the RT-PCR workflow through an extremely simplified genomic DNA removal step. (This one-step RT-PCR system is also available without ezDNase.)

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