规格
|
Sample Type:
|
Plasmid DNA |
Quantity:
|
1 mL |
Cell Type:
|
Established Cell Lines |
High-throughput Compatibility:
|
Not High-throughput Compatible (Manual) |
Serum Compatible:
|
Yes |
Transfection Technique:
|
Lipid-Based Transfection |
Product Line:
|
293fectin™ |
储存
|
Each unit contains one vial of reagent. Store at +4°C. 293fectin™ is guaranteed stable for 6 months when stored properly. |
293fectin™ Transfection Reagent is a proprietary, cationic lipid-based formulation for transfecting DNA into eukaryotic cells. This reagent is optimized for transfecting suspension 293 human embryonic kidney cells (e.g., FreeStyle™ 293-F cells, Cat. No. R790-07) in defined, serum-free FreeStyle™ 293 Expression Medium (Cat. No. 12338-018), and is intended for use with the FreeStyle™ 293 Expression System (Cat. No. K9000-01). 293fectin™ Transfection Reagent provides the following advantages:
• Demonstrates high transfection efficiency in suspension 293 cells and is also suitable for transfecting adherent 293 cells
• Suspension FreeStyle™ 293-F cells may be transfected in FreeStyle™ 293 Expression Medium; no medium change is required
• Add 293fectin™ reagent complexes directly to cells in culture medium
• No need to remove complexes or change or add medium following transfection