规格
Assay:	Tyrosine Kinase Assay
Assay Entry:	Biochemical phospho-specific activity (no antibody)
Excitation/Emission:	405⁄465, 535
No. of Reactions:	800 Reactions
Product Line:	Z´-LYTE®
Readout:	End Point
References:	Nature, 373, pp 536-9 (1995)
Shipping Condition:	Dry Ice
Source:	Abl Substrate (EAIYAAPF)
Substrate Properties:	Peptide-Based Substrate
Target Entry:	ABL1 G250E, ABL1 Y253F, RET Y791F, SYK, TYRO3, EPHB2, LYNA, MERTK, RET V804L, SRCN1, ABL1 E255K, EPHA4, FYN, RET, ABL1 T315I, FLT3, LCK, YES1, MET, ABL1, ABL2, FGR, HCK, EPHA1, EPHA8, EPHB3, LYNB, EPHB1, SRC, CSK
Target Enzyme:	Tyrosine Kinases
Technique:	FRET
Validated Kinases:	ABL1, ABL2, CSK2, EPHA1, EPHA4, EPHA8, EPHB1, EPHB2, MERTK, MET, RET, SRC, SRCN1, SYK, TYRO3, YES1, EPHB3, FGR, FLT, FYN, HCK, LCK, LYNA, LYNB
Label or Dye:	Methylcoumarin
Format:	384-well plate

储存
Z´-LYTE® Kinase Assay Kits contain all necessary reagents for performing primary or secondary screens. Most kit components should be stored at -20°C, and some kit components should be stored at -80°C. See COA for specific storage instructions.

描述

A coumarin and fluorescein-labeled peptide substrate which can be used for kinase screening in an antibody-free format. This peptide is based upon an optimal peptide substrate for ABL.

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