规格
|
Compatible Cells:
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Mammalian Cells, Eukaryotic Cells |
Excitation/Emission:
|
C12 Resazurin: 563⁄587, SYTOX® Green 504⁄523 |
Flow Cytometer Laser Lines:
|
488 |
Label Type:
|
Other Label(s) or Dye(s) |
Label or Dye:
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C12 Resazurin, SYTOX® Green |
No. of Reactions:
|
1,000 |
Product Line:
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LIVE/DEAD®, SYTOX® |
Shipping Condition:
|
Room Temperature |
Solubility:
|
DMSO (Dimethylsulfoxide) |
Detection Method:
|
Fluorescent |
For Use With (Equipment):
|
Fluorescence Microscope, Flow Cytometer, Microplate Reader |
Format:
|
Tube(s) |
储存
|
Contains 5 vials of C12-resazurin (40 µg each vial), 1 vial of SYTOX® green (100 µL, 10 µM solution in DMSO), 1 vial of DMSO (1.5 mL), and 10X phosphate buffer (100 mL).
(Store in freezer (-5 to -30°C) and protect from light. |
The LIVE/DEAD Cell Vitality Assay Kit provides a simple, two-color fluorescence assay that distinguishes metabolically active cells from injured cells and dead cells. The assay is based on the reduction of C12-resazurin to red-fluorescent C12-resorufin in metabolically active cells and on the uptake of the cell-impermeant, green-fluorescent nucleic acid stain, SYTOX Green dye, in cells with compromised plasma membranes (usually late apoptotic and necrotic cells). In this assay, dead cells emit mostly green fluorescence and healthy, metabolically active cells emit mostly red fluorescence; injured cells exhibit reduced red and green fluorescence.
View a selection guide for all Cell Viability Assays for Flow Cytometry.