规格
Column Type:	Agarose Resin, Affinity
Format:	15 mL conical, Kit, Spin Column
Product Line:	SulfoLink™
Stationary Phase:	Haloacetyl
Quantity:	5 column kit
Sufficient For:	Immobilizing 1 to 2mg of sulfhydryl-peptide per column

储存
Sufficient For: Immobilizing 0.1 to 1 mg of sulfhydryl-peptide per column • SulfoLink Columns, 2 mL, 5 columns • SulfoLink Coupling Buffer, 120 mL • Wash Solution, 120 mL • PBS Pack (makes 500 mL), 1 pack • Bond-Breaker TCEP Solution, Neutral pH, 0.5 mL • L-Cysteine·HCl, 100 mg • Column Accessories Store at 4°C protected from light.

描述

Thermo Scientific SulfoLink Immobilization Kit for Peptides, 2 mL is a kit containing all the reagents for preparing the peptide sample for efficient immobilization and five columns containing 2 mL of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification.

Features of SulfoLink Immobilization Kit for Peptides, 2 mL:

• Specific to sulfhydryl (-SH) groups—iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
• Fast—spin columns increase protocol speed; prepare and couple peptide samples in 2 hours
• Flexible coupling conditions—use pH 7.5 to 9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine·HCl), as needed for protein or peptide solubility during coupling reaction
• Easy-to-follow instructions—streamlined protocols for sample preparation, immobilization, and affinity purification
• High capacity—Immobilize 1 to 2 mg of peptide per 2 mL column of SulfoLink Coupling Resin

Applications:
• Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
• Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification

When incubated with a solution of peptide that contains reduced cysteine residues, the iodoacetyl groups of SulfoLink Resin react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.

Related Products
SulfoLink™ Coupling Resin
SulfoLink™ Immobilization Kit for Proteins, 2 mL
SulfoLink™ Immobilization Trial Kit, 2 mL

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