The Multi Tissue Dissociation Kits have been developed for the gentle yet effective isolation of different cell types from various tissues, including kidney, prostate, mouse embryo, and cell monolayer. There is a constant development of optimized tissue dissociation protocols for different tissue and cell types.

Please check available protocols in Details.

Background information

The Multi Tissue Dissociation Kits together with the gentleMACS™ Dissociators are recommended to obtain viable single-cell suspension from various tissues. The protocols were optimized for specific tissues and cell types for reliable results in downstream applications.

Applications

Multi Tissue Dissociation Kit 1:

This kit is recommended for the gentle dissociation of tissues while preserving cell surface epitopes. Therefore, it should be used when cell surface epitopes are essential for downstream applications, including cell separation, flow analysis and sorting.

Available protocols:

Dissociation of kidney (human)
Dissociation of prostate (mouse)
Dissociation of ear (mouse)
Dissociation of inflamed neural tissue

Multi Tissue Dissociation Kit 2:

The kit is recommend for the thorough dissociation of tissues. The kit is suitable for the isolation of cells with low frequency from dissociated tissues, such as endothelial cells.

Available protocols:

Dissociation of lung (rat)
Dissociation of kidney (mouse)
Dissociation of adult mouse heart
Dissociation of adult rat heart

Multi Tissue Dissociation Kit 3:

This kit is recommended for the gentle generation of single-cell suspensions from cell monolayers or mouse embryos. The dissociated cells are immediately ready for further cultivation.

Available protocols:

Dissociation of pluripotent stem cell (PSC) –derived cardiomyocytes
Dissociation of mouse embryos to generate mouse embryonic fibroblasts

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