- 商品介绍
- 规格参数
- 包装参数
产品介绍
Product Description
规格 |
|
HEPES Buffer: | No HEPES |
Product Line: | Gibco® |
Shipping Condition: | Room Temperature |
Sodium Pyruvate Additive: | Sodium Pyruvate |
Form: | Powder |
Glutamine: | L-Glutamine |
Phenol Red Indicator: | Phenol Red |
Quantity: | 10 x 1L |
储存 |
|
Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
描述 |
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco® F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This Ham's F-12 is modified as follows:
With | Without |
• L-glutamine | • Sodium Bicarbonate |
• Phenol Red |
The complete formulation is available.
Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.
cGMP Manufacturing and Quality System
Gibco® F-12 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Gibco® F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
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