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F 12 NUTRIENT MIX, HAMS 10X1L,21700075,Invitrogen

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订货号 4RK5612
品牌型号 Invitrogen 21700075
库存 有库存
最小订货量 1套
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产品介绍 Product Description
规格
HEPES Buffer: No HEPES
Product Line: Gibco®
Shipping Condition: Room Temperature
Sodium Pyruvate Additive: Sodium Pyruvate
Form: Powder
Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Quantity: 10 x 1L
储存
Storage conditions: 2-8° C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
描述

Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells. F-12 has since been used for serum-free growth of CHO cultures as well as serum-supplemented growth of other mammalian cells, including chondrocytes and rat prostate epithelial cells. We offer a variety of Gibco® F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This Ham's F-12 is modified as follows:

WithWithout
• L-glutamine• Sodium Bicarbonate
• Phenol Red

The complete formulation is available.

Compared to other basal media, F-12 contains a wider variety of components, including zinc, putrescine, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-12 has led to a variety of improved formulations.

cGMP Manufacturing and Quality System
Gibco® F-12 is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Gibco® F-12 contains no proteins or growth factors. Therefore, F-12 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-12 uses a sodium bicarbonate buffer system (1.176 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Powder forms of Gibco® cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

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