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LIVE/DEAD FUNGALIGHT YEAST VIA 1 KIT,L34952,Invitrogen

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订货号 4QT5825
品牌型号 Invitrogen L34952
货期 询货期
最小订货量 1套
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产品介绍 Product Description
规格
Compatible Cells: Yeast Cells
Detection Method: Fluorescent
Excitation/Emission: SYTO® 9: 485⁄498, PI: 535⁄617
Flow Cytometer Laser Lines: 488
Form: Solution
Format: Tube(s)
Label Type: Other Label(s) or Dye(s)
Label or Dye: SYTO® 9, propidium iodide
No. of Reactions: 200
Product Line: FungaLight™, LIVE/DEAD®
Shipping Condition: Room Temperature
Solubility: DMSO (Dimethylsulfoxide)
For Use With (Equipment): Flow Cytometer
储存
Contains 1 vial of SYTO® 9 dye (solution in DMSO), and 1 vial of propidium iodide, 300 μL (solution in DMSO).

Store in freezer (-5 to -30°C) and protect from light.
描述

The LIVE/DEAD® FungaLight™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO® 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy yeast cells. When used alone, the SYTO® 9 stain generally labels all yeast in a population - those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only yeast with damaged membranes, causing a reduction in the SYTO® 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present. As a result, yeast with intact membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.

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