规格
|
Compatible Cells:
|
Yeast Cells |
Detection Method:
|
Fluorescent |
Excitation/Emission:
|
SYTO® 9: 485⁄498, PI: 535⁄617 |
Flow Cytometer Laser Lines:
|
488 |
Form:
|
Solution |
Format:
|
Tube(s) |
Label Type:
|
Other Label(s) or Dye(s) |
Label or Dye:
|
SYTO® 9, propidium iodide |
No. of Reactions:
|
200 |
Product Line:
|
FungaLight™, LIVE/DEAD® |
Shipping Condition:
|
Room Temperature |
Solubility:
|
DMSO (Dimethylsulfoxide) |
For Use With (Equipment):
|
Flow Cytometer |
储存
|
Contains 1 vial of SYTO® 9 dye (solution in DMSO), and 1 vial of propidium iodide, 300 μL (solution in DMSO).
Store in freezer (-5 to -30°C) and protect from light. |
The LIVE/DEAD® FungaLight™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO® 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy yeast cells. When used alone, the SYTO® 9 stain generally labels all yeast in a population - those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only yeast with damaged membranes, causing a reduction in the SYTO® 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present. As a result, yeast with intact membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.