规格
Cloning Method:	TOPO®-TA
Key Functions:	Constitutive Expression
Product Line:	TOPO®, pcDNA™
Constitutive or Inducible System:	Constitutive
Delivery Type:	Transfection
Promoter:	CMV
Selection Agent (Eukaryotic):	Geneticin® (G-418)
Quantity:	20 reactions
Vector:	pcDNA
Protein Tag:	His Tag (6x), V5 Epitope Tag

储存

Each pcDNA™3.1 Directional TOPO¤ Expression Kit contains two boxes. The TOPO® box contains linearized and topoisomerase I-activated pcDNA3.1D/V5-His-TOPO® vector, dNTPs, control template and primers, primers for sequencing, and an expression control plasmid. Store at -20°C. The One Shot® box contains single-use 50-µl aliquots of One Shot® TOP10 Chemically Competent E. coli, S.O.C. medium, and pUC19 supercoiled plasmid control. Store at -80°C. Guaranteed stable for 6 months when properly stored.

描述

The pcDNA™3.1/V5-His TOPO® TA Expression Kit offers one-step cloning of Taq-amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1/V5-His-TOPO® vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants.

In addition, the vector includes the following features:

• Strong CMV promoter for high-level, constitutive expression.
• C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody.
• C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with an Anti-His (C-term) antibody.

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