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BIOTIN-14-DATP 50 NMOL,19524016,Invitrogen

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订货号 4MY2863
品牌型号 Invitrogen 19524016
货期 询货期
最小订货量 1套
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产品介绍 Product Description
规格
Label Type: Biotin & Other Haptens
Labeling Method: Direct Labeling
Label or Dye: Biotin
Shipping Condition: Approved for shipment on Wet or Dry Ice
Concentration: 0.4 mM
储存
Biotin-14-dATP is supplied at 0.4 mM in 100 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Store at -20°C.
描述

Biotin-14-dATP is a patented dATP analog with biotin attached at the 6-position of the purine base by a 14-atom linker. The long, 14-atom spacer arm allows accessibility to streptavidin for sensitive detection of probe-target hybrids in in situ and filter-based hybridizations. Biotin-14-dATP can be efficiently incorporated into DNA by nick translation in the presence of dCTP, dGTP, and dTTP (1). Biotin-14-dATP can be added to the 3´ end of DNA using terminal deoxynucleotidyl transferase (2). The amount supplied is sufficient for labeling up to 50 µg of DNA by nick translation.

Performance and Quality Testing: Purity is evaluated by reverse-phase HPLC analysis.

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