- 商品介绍
- 规格参数
- 包装参数
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规格 |
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| Final Product Type: | cDNA (First-Strand) |
| For Use With (Application): | Real-Time Quantitative PCR (qPCR) |
| Sample Type: | RNA |
| Quantity: | 2000 reactions |
| Size (Final Product): | 5 kb or less |
| Optimal Reaction Temperature: | 42° C |
| Reaction Format: | Separate Components |
| Reverse Transcriptase: | MultiScribe™ |
| Product Line: | TaqMan™ |
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储存 |
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Includes 10 units of Cat. No. N8080234. Each N8080234 kit contains: • 1 × 100 µL of MultiScribe™ Reverse Transcriptase (50U/µL) • 1 × 100 µL of RNase Inhibitor (20U/µL) • 1 × 1 mL of dNTP Mixture (2.5 mM each dNTP) • 1 × 100 µL of Oligo d(T)16 (50 µM) • 1 × 100 µL of Random Hexamers (50 µM) • 1 × 1.5 mL of 10X RT Buffer • 1 × 1.5 mL of MgCl2 Solution Reagents should be stored between -15°C and -25°C. A note about the size: This kit contains enough reagents for 2000 reverse transcriptase reactions when the reactions are set up at 10 µL each. |
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描述 |
TaqMan® Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility. Advantages of TaqMan® Reverse Transcription Reagents:
• Contain MultiScribe® Reverse Transcriptase, a recombinant moloney murine leukemia virus reverse transcriptase (MMLV RT)
• Random hexamers and oligo (dT)16 included for flexibility
• Individual components provide versatility in assay set-up
• Compatible with two-step RT-PCR reactions
Using TaqMan® Reverse Transcription Reagents
TaqMan® Reverse Transcription Reagents provide all the necessary components to perform the reverse transcription of RNA to cDNA. For two-step RT-PCR, random hexamers, oligo (dT)16, and sequence-specific reverse primers can all be used. The choice of primers for reverse transcription is best made after experimentally evaluating all three priming systems.
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¥ 10253.00
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