规格
Antibiotic Resistance Bacterial:	Ampicillin (AmpR)
Cloning Method:	Gateway®
Product Line:	Gateway®
Constitutive or Inducible System:	Inducible
Inducing Agent:	IPTG
Promoter:	T7 and lacO
Selection Agent (Eukaryotic):	None
Vector:	pET, pDEST
Protein Tag:	His Tag (6x), V5 Epitope Tag

储存
The Champion™ pET-DEST42 Gateway® destination vector includes 6 µg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.

描述

The Champion™ pET-DEST42 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. The Champion™ pET-DEST42 destination vector offers:

• Bacteriophage T7lac promoter for high-level expression
O operator sequences for lac repressor binding to ensure tighter regulation of transcription
• pBR322 ori for minimal basal expression
• C-terminal V5 and 6xHis tags for efficient detection and purification
R sites for efficient recombination with any attL-flanked Gateway® entry vector

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