Alexa Fluor® 488 Carboxylic Acid is a non-reactive form of Alexa Fluor® 488 that can be used as a reference standard for dye-conjugates. Additionally, this carboxylic acid derivative can be converted to an amine-reactive ester using standard chemical techniques or coupled to hydrazines, hydroxylamines, and amines in aqueous solution using water-soluble carbodiimides such as EDAC (Cat. No. E2247).

Characteristics of Alexa Fluor® 488 dye include:

• Laser line: 488 nm
• Common filter set: FITC (fluorescein)
• Excitation maxima: 500 nm
• Emission maxima: 525 nm

More About Alexa Fluor® 488 Dye
Alexa Fluor® 488 dye is a bright, green-fluorescent dye with excitation ideally suited to the 488 nm laser line. It is the brightest and most photostable green dye for sensitive detection in live or fixed cells. Alexa Fluor® 488 dye is commonly used with DAPI, and Alexa Fluor® 594 and 647 dyes for multiplexing. Learn more about this dye and related products.

Discover the Benefits of the Alexa Fluor® Dye Series
Compared to traditional dyes, Alexa Fluor® dyes are brighter, more photostable, and more pH-resistant between pH 4 and 10. And generally when using Alexa Fluor® dyes, higher degrees of labeling can be achieved without intramolecular quenching. For details see Alexa Fluor® Dyes Spanning the Visible and Infrared Spectrum in the Molecular Probes Handbook.

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