规格
For Use With (Application):	PCR, Southern Blotting, Sequencing, Cloning
Isolation Technology:	ChargeSwitch® Chemistry
Sample Type:	Bacteria
High-throughput Compatibility:	Not High-throughput Compatible (Manual)
Purification Target:	Genomic DNA
Shipping Condition:	Room Temperature
Product Line:	ChargeSwitch®

储存

All components of the ChargeSwitch® gDNA (genomic DNA) Mini Bacteria Kit are shipped at room temperature. Upon receipt, store components as follows: Store Proteinase K at 4“C. Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4“C. Store the remaining kit components at room temperature. All components are guaranteed stable for 6 months when stored properly.

Components:

ChargeSwitch® Magnetic Beads (25 mg/ml in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20)
ChargeSwitch® Resuspension Buffer (R4)
ChargeSwitch® Lysis Buffer (L14)
ChargeSwitch® Binding Buffer (B8)
ChargeSwitch® Wash Buffer (W12)
ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Proteinase K (20 mg/ml in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
RNase A (5 mg/ml in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA)

描述

The ChargeSwitch® gDNA Mini Bacteria Kit uses magnetic bead-based technology to provide rapid and efficient purification of up to 12 µg of genomic DNA from up to 0.5 ml of Gram-positive or Gram-negative bacterial culture (Figure 1) or an individual colony in less than 15 minutes after lysate preparation.
• Optimized for higher-purity DNA extraction
• Improved performance with accompanying magnetic rack
• Cleaner purified plasmid for optimal downstream performance

High Quality, High Yield Plasmid DNA
The ChargeSwitch® gDNA Mini Bacteria Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Genomic DNA is isolated without the need for hazardous chemicals, centrifugation, or vacuum manifolds.

Compatible with MagnaRack™ Magnetic Rack
The ChargeSwitch® gDNA Mini Bacteria Kit requires the use of a magnetic rack. We recommend using the MagnaRack™ Magnetic Rack, which is a two-piece magnetic separation rack that consists of a magnetic base station and a removable tube rack. The tube rack fits onto the magnetic base station in two different positions associating the row of 12 neodymium magnets with a single row of 12 tubes for simple and easy 'on the magnet' and 'off the magnet' sample processing.

Improved Performance in Downstream Applications
The ChargeSwitch® gDNA Mini Tissue Kit ensures reliable performance of the resulting gDNA in a variety of downstream applications, including PCR, restriction enzyme digestion, cloning, Southern blotting, and sequencing.

For Research Use Only. Not intended for diagnostic procedures.

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