- 商品介绍
- 规格参数
- 包装参数
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规格 |
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| For Use With (Application): | PCR, Southern Blotting, Sequencing, Cloning |
| Isolation Technology: | ChargeSwitch® Chemistry |
| Sample Type: | Bacteria |
| High-throughput Compatibility: | Not High-throughput Compatible (Manual) |
| Purification Target: | Genomic DNA |
| Shipping Condition: | Room Temperature |
| Product Line: | ChargeSwitch® |
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储存 |
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All components of the ChargeSwitch® gDNA (genomic DNA) Mini Bacteria Kit are shipped at room temperature. Upon receipt, store components as follows: Store Proteinase K at 4“C. Mix RNase A in Resuspension Buffer (R4) and store RNase A in Resuspension Buffer (R4) at 4“C. Store the remaining kit components at room temperature. All components are guaranteed stable for 6 months when stored properly. Components: ChargeSwitch® Magnetic Beads (25 mg/ml in 10 mM MES, pH 5.0, 10 mM NaCl, 0.1% Tween 20) ChargeSwitch® Resuspension Buffer (R4) ChargeSwitch® Lysis Buffer (L14) ChargeSwitch® Binding Buffer (B8) ChargeSwitch® Wash Buffer (W12) ChargeSwitch® Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5) Proteinase K (20 mg/ml in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol) RNase A (5 mg/ml in 10 mM Tris-HCl, pH 8.5, 10 mM EDTA) |
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描述 |
The ChargeSwitch® gDNA Mini Bacteria Kit uses magnetic bead-based technology to provide rapid and efficient purification of up to 12 µg of genomic DNA from up to 0.5 ml of Gram-positive or Gram-negative bacterial culture (Figure 1) or an individual colony in less than 15 minutes after lysate preparation.
• Optimized for higher-purity DNA extraction
• Improved performance with accompanying magnetic rack
• Cleaner purified plasmid for optimal downstream performance
High Quality, High Yield Plasmid DNA
The ChargeSwitch® gDNA Mini Bacteria Kit uses magnetic beads with a unique, ionizable functional group whose affinity for nucleic acids is pH-dependent to facilitate nucleic acid purification. Genomic DNA is isolated without the need for hazardous chemicals, centrifugation, or vacuum manifolds.
Compatible with MagnaRack™ Magnetic Rack
The ChargeSwitch® gDNA Mini Bacteria Kit requires the use of a magnetic rack. We recommend using the MagnaRack™ Magnetic Rack, which is a two-piece magnetic separation rack that consists of a magnetic base station and a removable tube rack. The tube rack fits onto the magnetic base station in two different positions associating the row of 12 neodymium magnets with a single row of 12 tubes for simple and easy 'on the magnet' and 'off the magnet' sample processing.
Improved Performance in Downstream Applications
The ChargeSwitch® gDNA Mini Tissue Kit ensures reliable performance of the resulting gDNA in a variety of downstream applications, including PCR, restriction enzyme digestion, cloning, Southern blotting, and sequencing.
For Research Use Only. Not intended for diagnostic procedures.
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瓶顶过滤器-500ml容量,MF75TM系列，聚苯乙烯外壳，75mmSFCA滤膜，孔径0.2um,适合瓶颈尺寸45mm，12/箱，291-4520，Nalgene，Thermofisher，赛默飞世尔询价
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