Stimulate T cells reactive to the JCV VP1 protein of the JC virus (JCV) using PepTivator Peptide Pools. PepTivators are pools of lyophilized peptides, consisting mainly of 15-mer sequences with 11 amino acids overlap. PepTivator^® JCV VP1 is a pool of lyophilized peptides, covering the sequence of the JCV VP1 protein (UniProtKB Acc. no. P03089).

The PepTivator is available in two different quality grades. The research-grade product has an average purity of 70%, whereas peptides of the premium-grade product are individually purified via HPLC having each a purity of >80%.

In vitro stimulation of antigen-specific T cells with PepTivator Peptide Pools causes the secretion of effector cytokines and the up-regulation of activation markers, which then allow the detection and isolation of antigen-specific T cells.

Background information

JCV is a widespread human polyomavirus with high homology to BK polyomavirus (BKV). Primary JCV infection results in asymptomatic latent persistence of the virus in the gastrointestinal tract and the kidney. Immunodeficiency or immunosuppression allows virus reactivation, which can cause, e.g., a fatal progressive multifocal leukoencephalopathy. VP1 is the major capsid protein expressed late in the lytic cycle. It is described to induce T cell immunity.

Downstream applications

PepTivator JCV VP1 has been specifically developed for the efficient in vitro stimulation of JCV VP1–specific T cells. Peptides of 15 amino acids in length and 11 amino acids overlap represent an optimized solution for stimulating both CD4⁺ and CD8⁺ T cells in various applications, including:

Detection and analysis of JCV VP1–specific effector/memory T cells in PBMCs by MACS^® Cytokine Secretion Assays, intracellular cytokine staining, or other technologies.
Isolation of viable JCV VP1–specific CD4⁺ T cells with the CD154 MicroBead Kit, or of CD4⁺ and CD8⁺ T cells using the CD137 MicroBead Kit or MACS Cytokine Secretion Assay – Cell Enrichment and Detection Kits. Subsequently, cells can be expanded for generation of T cell lines.
Generation of JCV VP1–specific effector/memory T cells from naive T cell populations.
Pulsing of antigen-presenting cells.

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