PIERCE AMINE-BINDING, MALEIC ANHYDRIDE ACTIVATED PLATES,，15100，赛默飞世尔

These Thermo Scientific Pierce Maleic Anhydride Activated 8-Well Strip Plates are clear, 96-well strip plates that allow attachment of amine-containing peptides and other molecules to microplate wells for use in binding assays.

Features of Maleic Anhydride Activated Plates:

• Spontaneously react with primary amines
• A direct, simple approach to covalently attach molecules to the well surface of an EIA plate
• Maleic anhydride maintains its integrity and availability for coupling for months under dry, room temperature conditions

These plates are useful for immobilizing peptides and other ligands that do not coat efficiently by passive adsorption. Reaction of the maleic anhydride groups with primary amines (-NH2) results in formation of amide bonds that are stable at neutral pH and above. Acidic conditions hydrolyze the bond; for example, the half-life of hydrolysis at pH 3.5 and 37°C is approximately 11 hours (Ref.1). Therefore, coating reactions are best performed at pH 8-9, and the reacted plates are best used at pH > 7 for ELISA and other methods.

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Pierce™ Maleic Anhydride Activated Plates, Clear, 96-Well
Pierce™ Maleic Anhydride Activated Plates, White, 96-Well