规格
|
Cloning Method:
|
Restriction Enzyme ⁄ MCS |
Product Line:
|
pVAX™ |
Constitutive or Inducible System:
|
Constitutive |
Delivery Type:
|
Transfection |
Promoter:
|
CMV |
Selection Agent (Eukaryotic):
|
None |
Vector:
|
pVAX1 |
Protein Tag:
|
Untagged |
储存
|
20 µg of each pVAX1 and expression control vector are supplied supercoiled and lyophilized. Store at -20°C. Vector is guaranteed stable for 6 months when properly stored. |
pVAX1 is specifically designed for use in the development of DNA vaccines. pVAX1 has the following features:
• Eukaryotic DNA sequences limited to those required for expression in order to minimize the possibility of chromosomal integration
• Kanamycin resistance gene for selection in E. colibecause ampicillin has been reported to cause an allergic response in some individuals
• Expression levels of recombinant proteins from pVAX1 comparable to those achieved with its parent vector, pcDNA™3.1
The small size of pVAX1 and the variety of unique cloning sites simplify subcloning of even very large DNA fragments.