- 商品介绍
- 规格参数
- 包装参数
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规格 |
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| Bacterial or Yeast Strain: | Mach1™ |
| Cloning Method: | Blunt TOPO® |
| Product Line: | One Shot™ |
| Vector: | Blunt DNA Cloning Vectors |
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储存 |
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Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO® vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot® Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
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描述 |
The Zero Blunt® TOPO® PCR Cloning Kit for subcloning combines the unique Zero Background™ technology with the pCR™- Blunt II-TOPO® vector (Figure 7) to allow easy, high-efficiency cloning of blunt-end PCR products.
• Unique technology to minimize background
• Get your clone—95% efficiency
• Multiple primer sites (T7, T3, M13F, M13R) make sequence analysis or PCR easy and convenient
• Kanamycin and Zeocin resistance genes for your choice of selection
• Convenient, validated restriction sites in MCS for further subcloning
• Includes MachI™ T1R competent cells for fast miniprep and shortens cloning time
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¥ 3498.00
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¥ 66363.43
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¥ 145427.01
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