规格
Final Product Type:	Total RNA
For Use With (Application):	Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Nuclease Protection Assays, Northern Blotting, cDNA Library Construction
Format:	Magnetic Bead
Isolation Technology:	ChargeSwitch® Chemistry
No. of Reactions:	50 Preps
Quantity:	5
Sample Type:	Cells, Bacteria
Yield:	40 µg
High-throughput Compatibility:	High-throughput Compatible, Automated Protocols
Purification Target:	Total RNA
Purification Time:	15 min
Scale:	Mini
Starting Material Amount:	Up to 10^6 cells
System Type:	ChargeSwitch®
Product Line:	ChargeSwitch®

储存
The ChargeSwitch® Total RNA Cell Kit includes ChargeSwitch® Magnetic Beads, Proteinase K, DNase I, DNase I Buffer, Lysis Buffer, Binding Buffer, Wash Buffers, and Elution Buffer. Store Proteinase K and Lysis Buffer at 4°C, DNase I at -20°C, and all remaining components at room temperature. All components are guaranteed stable for 6 months when stored properly.

描述

The ChargeSwitch® Total RNA Cell Kit is ideal for the purification of up to 25 µg of total RNA from up to 1 × 10⁶ cultured mammalian cells or from 1 ml overnight bacterial culture, including full-length RNA and small molecular weight RNA species. This kit offers a unique aqueous-based method for purifying total RNA from cultured mammalian or bacterial cells resulting in exceptionally clean and pure RNA with high integrity (Figure 1). ChargeSwitch® technology eliminates the use of common enzymatic inhibitors such as phenol, chaotropic salts or ethanol, resulting in exceptionally clean RNA. Due to its unique format, this kit also performs exceptionally well at concentrations useful for greater sensitivity in downstream analyses (Figure 3), yielding faster scientific results.

Features of The ChargeSwitch® Total RNA Cell Kit:

• Effectively captures up to 25 µg of total RNA from up to 1 × 10⁶ cultured mammalian cells or 1 ml bacterial culture
• Purifies a wide range of transcripts including increasingly important small RNAs (smaller than 200 nucleotides)
• Provides the ability to purify greater yields of RNA from very small sample sizes
• Eliminates the use of common enzymatic inhibitors providing exceptionally clean RNA
• Results in improved sensitivity in downstream analyses
• Allows flexibility for high-throughput applications

The ChargeSwitch® technology format is scalable and is a highly-sensitive RNA extraction method for successful downstream applications, including gene expression studies, promoter studies, siRNA, gene regulation, transcriptional analysis, RT-PCR, and qRT-PCR. This unique format also allows flexibility for application in high-throughput automation.

How ChargeSwitch® Technology Works:
Based on a patented switchable chemistry, ChargeSwitch® purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge attracting RNA. At higher pH, the surface chemistry is neutral, thereby releasing any attraction to bound RNA (Figure 2).

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