规格
|
Cell Line:
|
FreeStyle™ 293-F Cells |
Cell State:
|
Dividing Cells |
Druggable Target:
|
GPCRs |
Gene Symbol:
|
Gqi5 |
Product Line:
|
GeneBLAzer® |
Reporter Gene:
|
BLA (Beta-Lactamase) |
System Type:
|
GeneBLAzer™ |
Detection Method:
|
Fluorescent |
储存
|
Shipping: Dry Ice Storage: Liquid Nitrogen |
GeneBLAzer® Gqi5-NFAT-bla FreeStyle™ 293F cells contain the chimeric G-protein Gqi5 stably integrated into the CellSensor® NFAT-blaFreeStyle™ 293F cell line. CellSensor® NFAT-bla FreeStyle™ 293F cells contain a beta-lactamase reporter gene under control of a stably integrated NFAT response element. Gqo5-NFAT-bla FreeStyle™ 293F is a parental cell line designed to be used for the construction of Gi/o-coupled GPCR beta-lactamase cellular assays. The Gi/o receptor of interest should be stably expressed in the cell line by expression from the selectable vector pcDNA™6.2-hygro-DEST (Cat. no. K1233). Gqi5-NFAT-bla FreeStyle™ 293F cells have been shown to respond to the agonist of a transiently expressed D2 receptor.