规格
|
Product Line:
|
Pierce™ |
Crosslinker Type:
|
Homobifunctional |
Labeling Method:
|
Chemical Labeling |
Reactive Moiety:
|
Maleimide |
Solubility:
|
DMSO (Dimethylsulfoxide), DMF (Dimethylformamide) |
Spacer:
|
Cleavable, Long |
储存
|
Store desiccated at 4°C. Shipped at ambient temperature. |
Thermo Scientific Pierce DTME is a mid-length, maleimide crosslinker for reversible covalent conjugation between sulfhydryl groups (e.g., protein or peptide cysteines) by reduction of the disulfide bond in the center of the spacer arm.
Features of DTME:
• Reactive groups: maleimide (both ends)
• Reactive towards: sulfhydryl groups
• Long (13.3A), cleavable, sulfhydryl-to-sulfhydryl crosslinker, composed of maleimide groups and 11-atom disulfide spacer arm
• Water-insoluble—dissolve first in DMF or DMSO, then add to aqueous reaction buffers
• Cleavable by reduction of disulfide spacer arm with DTT, TCEP or other reducing agent
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
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